Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Article Title: Inhibiting activin-A signaling stimulates bone formation and prevents cancer-induced bone destruction in vivo.
doi: 10.1002/jbmr.142
Figure Lengend Snippet: Fig. 1. ActRIIA.muFc promotes osteoblastogenesis and increases bone mass. (A) Effect of activin-A, ActRIIA.muFc, and anti-activin-A antibody (mab3381) on mineralization. (B) TRACP-stained osteoclasts (blue arrows) and resorption pits (white arrows) in osteoclast cultures; addition of activin-A (0, 10, and 100 ng/mL) does not increase osteoclast number or bone resorption area. (C) Bone density of naive mice treated with increasing concentrations of ActRIIA.muFc at 6 weeks in the tibiaa. a ¼ p < .01 compared with vehicle. (D) mCT analysis of trabecular bone volume (BV/TV, %) and trabecular number (TB. N, /mm) of femurs of mice treated with ActRIIA.muFc. Lower, median, and higher quartiles are shown. b ¼ p < .001.
Article Snippet: Cells were incubated with vehicle (PBS), activin-A (20mg/m; R&D Systems, Minneapolis, MN, USA), ActRIIA.muFc (20mg/mL), or a monoclonal antibody against activin-A (mab3381, 10mg/mL; R&D Systems).
Techniques: Staining